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Chapters
- 00:05Title
- 00:57Sample Preparation
- 02:03Cell Disruption and Lipid Extraction
- 03:44Transesterification to fatty Acid Methyl Esters
- 04:51Quantification of Fatty Acid Methyl Esters Using Gas Chromatography
- 05:25Results: Fatty Acid Content and Composition of Microalgae as Determined by Gas Chromatography
- 07:19Conclusion
A method for the determination of fatty acid content and composition in microalgae based on mechanical cell disruption, solvent based lipid extraction, transesterification, and quantification and identification of fatty acids using gas chromatography is described. A tripentadecanoin internal standard is used to compensate for the possible losses during extraction and incomplete transesterification.
