Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, <i>Hirudo medicinalis</i>

Josh Titlow; Zana R. Majeed; John G Nicholls; Robin L. Cooper

doi:10.3791/50631

Journal

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신경과학

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Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

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신경과학

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Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

DOI:

10.3791/50631-v

•

11:53 min

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November 04, 2013

•

Josh Titlow, Zana R. Majeed², John G Nicholls, Robin L. Cooper

¹Department of Biology,University of Kentucky, ²Department of Biology, College of Science,University of Salahaddin, Iraq, ³Department of Neurobiology and Cognitive Neuroscience,SISSA, Italy

Chapters

00:05Title
01:30An Introduction from John Nicholls
03:59Ventral Ganglion Dissection
05:36Culturing Neurons from the Ventral Ganglion Preparation
07:26Ganglion-body Wall Preparation
08:46Interpreting Recordings and Making Dye Injections
11:07Conclusion

Summary

자동 번역

This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.

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