Journal
/
/
无标记的分离和细胞的富集通过非接触式介电泳
JoVE 신문
의학
This content is Free Access.
JoVE 신문 의학
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
DOI:

10:38 min

September 03, 2013

, , ,

Chapters

  • 00:05Title
  • 01:54Fabricating a Prototype cDEP Microfluidic Device
  • 03:12Preparing a Cell Suspension in DEP Buffer
  • 03:57Loading the cDEP Microfluidic Chip
  • 06:04Characterizing Crossover Frequency of Cells Using cDEP Chip
  • 07:49Varying the Experiment to Perform Cell Sorting or Enrichment
  • 08:43Results: Cell Response and Sorting by Contactless Dielectrophoresis
  • 09:50Conclusion

Summary

자동 번역

非接触式介电电泳(CDEP)达到通过其固有的介电性能和分拣颗粒的富集。流体通道的电极取代金属电极的传统,以DEP,适合CDEP非破坏无菌表征和生物粒子进行排序。我们将演示如何编写一个CDEP微型设备,并进行细胞鉴定和分选实验。

Related Videos

Read Article