Forward Genetic Approaches in <em>Chlamydia trachomatis</em>

00:05Title
01:20Chemical Mutagenesis
03:49Clonal Isolation of Chlamydia trachomatis Mutant Strains by Plaque Purification
07:14Representative Chlamydia Mutagenesis Results
08:29Conclusion

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We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.

Forward Genetic Approaches in Chlamydia trachomatis

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