JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:20Neonatal Microglia Dissection: Day 0
- 04:24Cell Culture: Day 0
- 05:31Cell Culture: Day 10
- 06:40Adult Microglia: Tissue Colection
- 07:56Digestion of Spinal Cord Tissue
- 09:07Results: Representative Images of Neonatal and Adult Microglia
- 10:50Conclusion
We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.
