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Anticorps coloration dans<em> C. Elegans</em> Utilisation de «gel-Cracking"
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JoVE 신문 생물학
Antibody Staining in C. Elegans Using "Freeze-Cracking"
DOI:

13:10 min

October 14, 2013

Chapters

  • 00:05Title
  • 01:13Preparation of Frozen Nematode Slides
  • 04:17Preparation of Slides of Individual Nematodes
  • 05:32Fixation: Light Fix of Methanol-Acetone
  • 06:30Fixation: Hard Fix Using Formaldehyde or Glutaraldehyde
  • 08:22Preparation of Slides for Microscopy
  • 10:32Representative Images
  • 12:45Conclusion

Summary

자동 번역

"Freeze-fissuration," une méthode pour exposer les tissus internes du nématode C. elegans aux anticorps pour la localisation des protéines, est démontrée.

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