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Chapters
- 00:05Title
- 01:32Microscope Alignment
- 03:14Laser Alignment
- 05:14Measurement of Readout and Activation Beam Profiles
- 06:40Image Acquisition: Multicolor FPALM
- 10:08Results: Identifying Spatial Distributions of Proteins Using FPALM
- 12:13Conclusion
We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single cells.
