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Chapters
- 00:05Title
- 01:14Growing Bacterial Host (BLT5403) for Titering
- 02:15Affinity Selection
- 08:13Titering
- 09:41Plaque Isolation
- 10:59Results: Typical Titer and PCR Results for Affinity Selection over an Appropriate Bait
- 12:12Conclusion
Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of interactors.
