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Chapters
- 00:05Title
- 02:04Longitudinal Muscle/Myenteric Plexus Harvesting
- 04:42LMMP Digestion
- 07:18Results: An in vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
- 09:16Conclusion
We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.
