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整个老鼠大脑与光共聚焦显微镜板微米级分辨率光学层析成像

JoVE 신문
신경과학

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JoVE 신문 신경과학

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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整个老鼠大脑与光共聚焦显微镜板微米级分辨率光学层析成像

DOI:

10.3791/50696-v

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09:49 min

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October 08, 2013

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Ludovico Silvestri, Alessandro Bria³, Irene Costantini, Leonardo Sacconi⁴, Hanchuan Peng, Giulio Iannello, Francesco Saverio Pavone^4,6,7

¹European Laboratory for Non-linear Spectroscopy (LENS), ²Integrated Research Centre,University Campus Bio-medico of Rome, ³DAEMI,University of Cassino, ⁴National Institute of Optics (CNR-INO), ⁵Allen Institute for Brain Science, ⁶Department of Physics,University of Florence, ⁷ICON Foundation, Sesto Fiorentino, Italy

Chapters

00:05Title
01:22Dehydration and Clearing
02:37Specimen Mounting
03:33Tomography Preparation
04:26Tomography Acquisition
05:37Stitching and Visualization
07:35Results: Micron-scale Resolution Optical Tomography
08:54Conclusion

Summary

자동 번역

在这篇文章中，我们描述了完整的实验程序来重建，具有分辨率高，荧光标记的小鼠大脑的优良大脑解剖。所描述的协议包括样品制备和清算，样品安装成像，数据后处理和多尺度可视化。

Tags

Keywords: Optical Tomography Confocal Light Sheet Microscopy CLSM Entire Mouse Brain Micron-scale Resolution Fluorescent Proteins EGFP L7-GFP Thy1-GFP-M Whole Brain Imaging Neuronal Soma Neuronal Projections Data Management Stitching Multi-resolution Navigation

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