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Chapters
- 00:05Title
- 01:11Harvesting and Preparation of Adipose Tissue
- 02:06Collagenase Digestion
- 03:19Staining of Cell Surface Antigens
- 04:23FACS Analysis
- 05:13Representative Results
- 06:54Conclusion
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
