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、検出し可視化し、アメーバモデル系で活性酸素種の生成を定量
JoVE 신문
생물학
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JoVE 신문 생물학
Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
DOI:

16:41 min

November 05, 2013

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Chapters

  • 00:05Title
  • 02:42OxyBurst Green (OBG)-Coating of Beads
  • 05:43Visualization and Qualitative Measurement of ROS Production in Phagosomes
  • 08:48Quantitative and Medium-throughput Measurement of Intracellular Superoxide Production
  • 10:30Quantitative and Medium-throughput Measurement of Extracellular H2O2 Production
  • 12:04Results: OxyBurst Green (OBG), Dihydroethidium (DHE) and Amplex UltraRed (AUR) Assays can Detect and Measure ROS in Dictyostelium
  • 15:54Conclusion

Summary

자동 번역

我々は、定性的および定量的研究のための様々なアメーバおよび哺乳動物細胞モデルに適用することができる反応性酸素種(ROS)の測定のためのプロトコルのセットを適合。

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