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Chapters
- 00:05Title
- 01:27Environmental Control Conditions for Live-cell Fluorescent Microscopy
- 03:44Real-time Imaging of Anterograde Virion Transport Events
- 06:43Overnight Time-lapse Imaging of Anterograde Spread Events
- 09:08Image Processing and Export
- 10:48Results: Representative Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
- 15:06Conclusion
Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.
