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Chapters
- 00:05Title
- 01:52Seed Sterilization
- 03:14Seed Plating
- 04:03Seed Dissection
- 05:29Assembly of the Seed Coat Bedding Assay (SCBA)
- 06:33Results: Representative Seed Material Unable to Synthesize Gibberellins
- 08:10Conclusion
We present the procedure to assemble a seed coat bedding assay (SCBA) from Arabidopsis thaliana seeds. The SCBA was shown to be a powerful tool to explore genetically and in vitro how the endosperm controls seed germination in dormant seeds and in response to light cues. The SCBA is in principle applicable under any situation where the endosperm is suspected to influence embryonic growth.
