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肠道细菌侵入肠上皮细胞<em>体外</em>使用垂直扩散室模型，显着增强

JoVE 신문
면역학 및 감염병학

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Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

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肠道细菌侵入肠上皮细胞<em>体外</em>使用垂直扩散室模型，显着增强

DOI:

10.3791/50741-v

•

10:52 min

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October 22, 2013

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Neveda Naz, Dominic C. Mills, Brendan W. Wren, Nick Dorrell

¹Faculty of Infectious & Tropical Diseases,London School of Hygiene & Tropical Medicine

Chapters

00:05Title
01:47Growth of Intestinal Epithelial Cell (IEC) Monolayers on 0.4 µm Filters
02:22Vertical Diffusion Chamber (VDC) Co-culture Assay Preparation
04:21Setting up of the VDC
06:14Disassembling of the VDC after Co-culture
08:34Results: The VDC Model Facilitates Greater Numbers of Bacterial-IEC Interactions and Invasions than Standard Cell Culture Assay Conditions
10:07Conclusion

Summary

자동 번역

在有氧条件下进行细胞培养实验调查细菌的粘附和侵袭，通常是不具代表性的<em>在体内</em>环境。 A垂直扩散室模型允许人类病原体的相互作用研究<em>空肠弯曲菌</em与肠上皮细胞在更<em>在体内</em>如条件，从而增强细菌的侵袭。

Tags

Vertical Diffusion Chamber In Vitro Model Intestinal Epithelial Cells Campylobacter Jejuni Microaerobic Conditions Host-pathogen Interactions Bacterial Invasion Cell Culture

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