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JoVE Journal Chemistry
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
 
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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Article DOI: 10.3791/50747-v • 14:44 min • December 16th, 2013
December 16th, 2013 •
1, 1, 2
1Department of Chemistry, The Hebrew University of Jerusalem, 2Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem

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The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

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Keywords: Peptide-metal Complexes Copper (I) Binding Metallochaperone Transport Proteins NMR Spectroscopy 1D And 2D 1H NMR Structure Determination Molecular Mechanics Calculations Solution Structure Unbiased Structure Determination
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