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Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
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Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

DOI:
10.3791/50757-v

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09:18 min

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September 05, 2013

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, ,
1Graduate Program of Environmental Science,Arkansas State University, 2Department of Biological Sciences,Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology,Arkansas State University

Chapters

  • 00:05Title
  • 01:26Tissue Treatment and Lipid Extraction
  • 02:33Lipid Separation by Preparative Thin-layer Chromatography
  • 05:34TAG Analysis by MALDI-TOF MS
  • 07:21Results: Triacylglyceride Profiles of Eastern Red Bat Tissues
  • 08:30Conclusion

Summary

자동 번역

자동 번역

Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Tags

TriacylglyceridesSebumLipid CompositionThin-layer ChromatographyMALDI-TOF Mass SpectrometryMammalian IntegumentInnate Immune SystemSkin Diseases

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