/
/
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:12Human Respiratory Syncytial Virus (RSV) A2 Culture
- 02:39RSV Purification
- 04:14Peripheral Blood Mononuclear Cell (PBMC) Isolation from Healthy Volunteer Blood
- 05:50Stimulation of PBMC with Live RSV
- 06:30Results: Representative Human PBMC Immune Responses to RSV Infection
- 08:26Conclusion
Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection.
