JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:23Pre-immunohistochemistry
- 03:44Heat-induced Antigen Retrieval
- 04:52Primary and Secondary Antibody Staining
- 06:50Results: Development of Neural Stem Cell During Adult Hippocampal Neurogenesis
- 08:27Conclusion
During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.
