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Chapters
- 00:05Title
- 01:48Preparation of GST and Nanoparticle Conjugates
- 03:48Surface Preparation
- 05:07Monitoring On-chip Capture-cycloaddition of Functionalized Molecules
- 06:26Monitoring Immobilization Density and Determination of Cycloaddition Rates
- 08:27Measuring the Binding of FKBP12 to Immobilized AP1497
- 10:49Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance
- 13:51Conclusion
Se presenta un método para la rápida inmovilización reversible de moléculas pequeñas y las asambleas de nanopartículas funcionalizadas de resonancia de plasmones superficiales (SPR) los estudios, utilizando secuencial en el chip de captura química cicloadición bioorthogonal y anticuerpo-antígeno.
