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Mikroflödes On-chip Capture-cykloadditionsreaktion att reversibelt Immobilisera små molekyler eller flerkomponent Strukturer för Biosensor Applications
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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
DOI:

14:43 min

September 23, 2013

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Chapters

  • 00:05Title
  • 01:48Preparation of GST and Nanoparticle Conjugates
  • 03:48Surface Preparation
  • 05:07Monitoring On-chip Capture-cycloaddition of Functionalized Molecules
  • 06:26Monitoring Immobilization Density and Determination of Cycloaddition Rates
  • 08:27Measuring the Binding of FKBP12 to Immobilized AP1497
  • 10:49Results: Real-time Monitoring of On-chip Small Molecule Immobilization by Surface Plasmon Resonance
  • 13:51Conclusion

Summary

자동 번역

Vi presenterar en metod för snabb, reversibel immobilisering av små molekyler och funktionnanopartiklar församlingar för ytplasmonresonans (SPR) studier, med hjälp av sekventiell on-chip bioorthogonal cykloadditionen kemi och antikropp-antigen-capture.

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