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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
 
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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Article DOI: 10.3791/50773-v • 06:33 min • December 9th, 2013
December 9th, 2013 •
1,2, 1,2, 1,2, 1,2
1Université Claude Bernard Lyon, 2Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534

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This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

Tags

DNA Cytoplasm Syncytial Gonad Caenorhabditis Elegans Transgenic Lines Extrachromosomal Arrays Transmission Rate Mosaic UV Irradiation Nonmosaic Transgenic Strains Mendelian Transmission Rate F1 Progenies Heterozygous Integration F2 Progeny Transgene Transmission Population Nonintegrated Transgenic Lines
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