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電子プローブX線マイクロアナリシスによるニューロンにおける総カルシウムの測定
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JoVE 신문 신경과학
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
DOI:

11:42 min

November 20, 2013

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Chapters

  • 00:05Title
  • 01:29Rapid Freezing and Cryosectioning of Cells Cultured on Coverslips
  • 03:33Cryotransfer of Specimens to the Electron Microscope
  • 05:00Freeze Drying and Imaging of Cells and Organelles
  • 06:35Acquisition of Mitochondrial X-ray Spectra
  • 07:44Qualitative and Quantitative Analysis of X-ray Spectra
  • 09:33Results: Mitochondria Sequester Calcium in Response to Glutamate Receptor Activation
  • 11:17Conclusion

Summary

자동 번역

本稿では、生理学的に定義された生物試料中の細胞下解像度で、全カルシウム含有量や分布を定量的に測定するcryoanalytical電子顕微鏡の応用について説明しています。

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