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Chapters
- 00:05Title
- 02:44Yeast Luminometry Method
- 04:23cRNA Injection
- 06:08Two-electrode Voltage-clamp
- 08:11Patch Clamp Examination of Direct Mechanosensitivity
- 09:41Results: Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
- 11:38Conclusion
Ter aanvulling gebruikte methoden bestuderen TRPV4's worden twee methoden beschreven: De mechanische gevoeligheid kan worden bestudeerd door Ca 2 +-aequorine luminometrie in transgene gist bij hypo-osmotische uitdaging. Het kan ook in TRPV4-RNA worden onderzocht geïnjecteerd Xenopus oöcyten door whole-cell twee-elektrode voltage clamp of patch klem in op cel-of uitgeschakelde modus.
