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The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
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The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

DOI:
10.3791/50824-v

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07:47 min

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November 13, 2013

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,
1Department of Craniofacial Development and Stem Cell Biology, and Department of Orthodontics, Dental Institute, Guy’s Hospital, UK,King’s College London, 2Department of Pediatric Dentistry and Orthodontics, College of Dentistry,King Saud University, Kingdom of Saudi Arabia

Chapters

  • 00:05Title
  • 01:08Embryo Dissection
  • 02:07Embryo Slicing
  • 04:14Mandible Slice Culture and Lineage Tracing
  • 06:47Documenting Early Stages of Tooth Development
  • 07:22Conclusion

Summary

자동 번역

자동 번역

Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.

Tags

Explant CultureSlice CultureTooth Germ DevelopmentOrgan Culture발생학MorphogenesisLineage Tracing

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