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Chapters
- 00:05Title
- 01:40Making the Poly(dimethylsiloxane) (PDMS) Layer
- 02:29Assembling the Flow Cell
- 03:41Loading the Bacterial Suspension
- 05:08Loading the Solutions
- 06:15Image Acquisition
- 08:02Disinfecting the Flow Cell and Counting the Bacteria
- 09:21Results: Representative Bacterial Susceptibility Imaging and Survival Data
- 12:07Conclusion
We have developed a microfluidic platform for rapid antibiotic susceptibility testing. Fluid is passed at high speeds over bacteria immobilized on the bottom of a microfluidic channel. In the presence of stress and antibiotic, susceptible strains of bacteria die rapidly. However, resistant bacteria can survive these stressful conditions.
