High Throughput Microinjections of Sea Urchin Zygotes

Nadezda A. Stepicheva; Jia L. Song

doi:10.3791/50841

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High Throughput Microinjections of Sea Urchin Zygotes

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High Throughput Microinjections of Sea Urchin Zygotes

DOI:

10.3791/50841-v

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12:40 min

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January 21, 2014

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Nadezda A. Stepicheva, Jia L. Song

¹Department of Biological Sciences,University of Delaware

Chapters

00:05Title
01:26Experimental Preparations
04:00Collecting Sea Urchin Gametes
06:12Rowing the Eggs
07:40Microinjection of the Sea Urchin Zygotes
11:13Injection of Reporter Constructs Does Not Affect Development
12:05Conclusion

Summary

자동 번역

Microinjection is a common technique used to deliver DNA constructs, mRNAs, morpholino antisense oligonucleotides or other treatments into eggs, embryos, and cells of various species.

High Throughput Microinjections of Sea Urchin Zygotes

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Chapters

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