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海胆受精卵的高通量显微注射

JoVE 신문
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High Throughput Microinjections of Sea Urchin Zygotes

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海胆受精卵的高通量显微注射

DOI:

10.3791/50841-v

•

12:40 min

•

January 21, 2014

•

Nadezda A. Stepicheva, Jia L. Song

¹Department of Biological Sciences,University of Delaware

Chapters

00:05Title
01:26Experimental Preparations
04:00Collecting Sea Urchin Gametes
06:12Rowing the Eggs
07:40Microinjection of the Sea Urchin Zygotes
11:13Injection of Reporter Constructs Does Not Affect Development
12:05Conclusion

Summary

자동 번역

显微注射是用于递送DNA构建体，mRNA表达，吗啉代反义寡核苷酸或其它处理成卵，胚胎，以及不同物种的细胞中的常见技术。

Tags

Keywords: Microinjection Sea Urchin Zygotes High-throughput DNA Constructs MRNAs Recombinant Proteins Gain Of Function Loss Of Function Fluorescent Dye Colorimetric Dye Cell Delivery Embryo Delivery Biological Processes

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