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JoVE Journal
Biology

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FtsZ Polymerization Assays
 
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FtsZ Polymerization Assays: Simple Protocols and Considerations

Article DOI: 10.3791/50844-v 12:04 min November 16th, 2013
November 16th, 2013

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Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.

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Keywords: FtsZ Polymerization Cell Division GTP Hydrolysis Sedimentation Light Scattering Electron Microscopy Buffer Conditions Escherichia Coli Bacillus Subtilis
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