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与器官型脑片和癌细胞的3D球体共培养系统

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Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

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与器官型脑片和癌细胞的3D球体共培养系统

DOI:

10.3791/50881-v

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07:48 min

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October 09, 2013

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Han-Ning Chuang, Raphaela Lohaus, Uwe-Karsten Hanisch, Claudia Binder, Faramarz Dehghani*³, Tobias Pukrop*¹

¹Department of Hematology and Oncology,University of Göttingen, ²Institute of Neuropathology,University of Göttingen, ³Institute of Anatomy and Cellbiology,University of Halle

Chapters

00:05Title
01:41Organotypic Brain Slice Coculturing
04:11Immunofluorescence and Imaging of Astrocytes and Microglia
05:42Representative Coculturing Results
07:11Conclusion

Summary

자동 번역

在器官型脑切片的共培养与肿瘤细胞使肿瘤细胞侵袭的脑组织的过程中可视的形态变化用荧光以及明场（视频）显微镜。这个模型系统还允许细胞交换和补充的方法，并提供多种操作和分析。

Tags

Keywords: Coculture Organotypic Brain Slice 3D Spheroid Carcinoma Cells Cerebral Metastasis Tumor Microenvironment Tumor-associated Macrophages (TAM)Astrocytes In Vitro Model Brain Colonization Glial Reactions Molecular Analysis

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