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Chapters
- 00:05Title
- 01:38Sample Washing
- 02:15Sample Grinding: Method 1 (Large Samples)
- 02:55Cortisol (CORT) Extraction: Method 1 (Large Samples)
- 03:41Sample Grinding: Method 2 (Small Samples)
- 04:24CORT Extraction: Method 2 (Small Samples)
- 05:14Solvent Evaporation, Sample Reconstitution, and CORT Assay
- 05:51Results: Representative CORT Extraction Analysis for Human Hair Samples
- 08:01Conclusion
Cortisol (CORT) accumulates in the growing hair shaft of humans and nonhuman primates. We describe methods for extracting and analyzing hair CORT with high precision and sensitivity. Measurement of hair CORT is particularly well-suited for assessing chronic stress over periods of weeks to months.
