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Journal / Neuroscience / Laser-scanning Photostimulation of…
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Neuroscience

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JoVE Journal Neuroscience
Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits
 
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Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Article DOI: 10.3791/50915-v • 07:43 min • December 27th, 2013
December 27th, 2013 •
1, 2, 1, 2
1Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 2Department of Neurobiology, University of Chicago

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We describe a method for characterizing the functional topography and synaptic properties of forebrain circuits using an optogenetic approach to photostimulate neuronal populations in vitro.

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Keywords: Laser-scanning Photostimulation Optogenetics Forebrain Circuits Channelrhodopsin Cre-lox Recombination Whole-cell Patch Clamp Functional Topography Synaptic Properties Confocal Microscopy
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