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Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
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Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

DOI:
10.3791/50933-v

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06:43 min

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December 07, 2013

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, , , ,
1Department of Biochemistry and Biomedical Sciences,McMaster University, 2Thrombosis and Atherosclerosis Research Institute,McMaster University

Chapters

  • 00:05Title
  • 01:01Harvesting Heart and Aorta and Preparation of Aorta
  • 02:13Sectioning the Aorta
  • 03:09Staining and Quantification of Atherosclerotic Lesion
  • 04:44Representative Results
  • 06:18Conclusion

Summary

자동 번역

자동 번역

We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.

Tags

AtherosclerosisAortic SinusMurine ModelLesion QuantificationCross-sectional AreaLesion VolumeValve LeafletsAnatomical LandmarkStaining MethodsAtherogenesis

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