/
/
Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:28Isolation of Newborn Inguinal Lymph Nodes (LN)
- 02:38Isolation of E18.5 Fat Pads
- 03:28Generation of the LN-fat Pad Chimera
- 05:14Isolation and Enzymatic Digestion of the Transferred LN for Flow Cytometric Analysis
- 06:31Analysis of the Transferred LNs by Immunofluorescence
- 07:16Results: Typical Experimental Results Obtained Using the LN/fat Pad Chimera Method
- 08:44Conclusion
Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.
