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A Novel Tre-dimensionel Flow Chamber Device at studere Chemokin-rettet Ekstravasation Celler Cirkulerende under fysiologiske strømning
JoVE 신문
생체공학
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JoVE 신문 생체공학
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

A Novel Tre-dimensionel Flow Chamber Device at studere Chemokin-rettet Ekstravasation Celler Cirkulerende under fysiologiske strømning

DOI:
10.3791/50959-v

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10:56 min

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July 15, 2013

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,
1Torrey Pines Institute for Molecular Studies, 2Cascade LifeSciences Inc.

Chapters

  • 00:05Title
  • 02:14Collagen Precoating of Microporous Inserts
  • 03:44Culture of Endothelial Cells on the Upper Inserts
  • 04:573D Flow Chamber Assembly and Priming
  • 07:59Circulating Cell Assay and Sample Collection
  • 09:19Results
  • 10:26Conclusion

Summary

자동 번역

자동 번역

Det tredimensionale strømningskammeret enhed er en roman<em> In vitro</em> Teknologi til kvantitative og trinvis evaluering af ekstravasation kaskade af celler cirkulerer under betingelser med fysiologisk shear stress. Enheden derfor udfylder et kritisk behov for grundlæggende, prækliniske og kliniske undersøgelser af celle migration.

Tags

Keywords: 3D Flow ChamberCell ExtravasationShear StressChemokine GradientEndothelial CellsMicroenvironmentCell MigrationDrug ScreeningIn Vitro Model

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