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使用<em>大蜡螟</em>作为模式生物来研究<em>嗜肺军团菌</em>感染

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection

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使用<em>大蜡螟</em>作为模式生物来研究<em>嗜肺军团菌</em>感染

DOI:

10.3791/50964-v

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12:30 min

•

November 22, 2013

•

Clare R. Harding, Gunnar N. Schroeder, James W. Collins, Gad Frankel

¹Center for Molecular Bacteriology and Infection,Imperial College London

Chapters

00:05Title
01:38Preparation of L. pneumophila for Infection
03:04Preparation and Infection of G. mellonella
05:39Analysis of Larval Mortality
06:17Extraction and Processing of Hemolymph
08:57Quantifying the Bacterial CFU
09:37Virulence of WT and Dot/Icm-deficient L. pneumophila
12:07Conclusion

Summary

자동 번역

蜡蛾蜡螟的幼虫最近被确立为一种体内模型来研究嗜肺军团菌感染。在这里，我们展示了基本技术， 军团菌的发病特点的幼虫，包括接种，细菌毒力和复制的测量和提取被感染的血细胞和分析。

Tags

Keywords: Galleria Mellonella Legionella Pneumophila Infection Model Hemocytes Phagocytes Dot/Icm Type IV Secretion System Legionella Containing Vacuole Bacterial Virulence Competition Assays

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