Journal
/
화학
/
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

DOI:
10.3791/50969-v

•

10:02 min

•

February 18, 2014

•

,
1Department of Chemistry and Biochemistry,Florida International University

Chapters

  • 00:05Title
  • 01:42Experimental Setup
  • 05:18PBD Measurements
  • 06:28Data Analysis
  • 08:17Results: Representative PBD Traces
  • 09:28Conclusion

Summary

자동 번역

자동 번역

Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

Tags

Keywords: Photothermal Beam DeflectionPhoto-acoustic CalorimetryThermal GratingConformational ChangesProteinsMicrosecond To Millisecond Time-scalesVolume ChangesEnthalpy ChangesCalcium BindingCalcium TransducersNeuronal Calcium SensorsDM-nitrophenPhoto-triggeringFast Kinetics

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/50969?article_type=v

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
check_url/kr/50969?article_type=v

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
check_url/kr/50969?article_type=v

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
check_url/kr/50969?article_type=v

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
check_url/kr/50969?article_type=v

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
check_url/kr/50969?article_type=v

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
check_url/kr/50969?article_type=v

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
check_url/kr/50969?article_type=v

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
check_url/kr/50969?article_type=v

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−
check_url/kr/50969?article_type=v

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Read Article