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隔离细胞脂滴：两个纯化技术从酵母细胞与人类的胎盘开始

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Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

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隔离细胞脂滴：两个纯化技术从酵母细胞与人类的胎盘开始

DOI:

10.3791/50981-v

•

09:41 min

•

April 01, 2014

•

Jaana Mannik*¹, Alex Meyers*², Paul Dalhaimer²

¹Department of Biochemistry and Cellular and Molecular Biology,University of Tennessee, ²Department of Chemical and Biomolecular Engineering,University of Tennessee

Chapters

00:05Title
01:01Preparing Placenta for Dissection
04:49Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
07:43Results: Isolation of Lipid Droplets from Placental Villous Cells
09:16Conclusion

Summary

자동 번역

两种技术从1分离细胞的脂滴），酵母细胞和2）人类胎盘介绍。这两个程序的核心是密度梯度离心法，在这里可以用肉眼很容易地可视化含有液滴产生的浮层，提取，并通过Western印迹分析纯度量化。

Tags

Lipid Droplets Density Gradient Centrifugation Yeast Cells Human Placental Villous Cells Protein Isolation Proteomic Analysis Lipidomic Analysis

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