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使用微流体芯片实时成像和损伤研究的响应<em>果蝇</em>幼虫
JoVE 신문
생체공학
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JoVE 신문 생체공학
Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae
DOI:

11:46 min

February 07, 2014

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Chapters

  • 00:05Title
  • 01:47Preparing the PDMS Chip
  • 03:47Using the Larva Chip for Live Imaging
  • 07:23Inducing a Nerve Crush Injury to Larval Segmental Nerves
  • 08:42Immobilization Enhanced Imaging
  • 10:25Conclusion

Summary

자동 번역

果蝇幼虫对于实时成像,由于其半透明的角质层和强大的遗传学一个有吸引力的模型系统。本协议描述了如何利用单层PDMS设备,被称为“幼虫芯片的细胞过程的3 幼虫果蝇的神经元内的实时成像。

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