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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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Chapters
- 00:05Title
- 01:32Plant Growth and Target Gene Silencing
- 03:22Preparation of Nonhost Pathogen Cultures of Plant Inoculation
- 04:26Observation of in planta Growth of Bacterial Pathogens
- 05:13Sequencing the Insert and Identification of Target Gene
- 05:50Results: Gene-silenced N. benthamiana Plants Compromised for Nonhost Resistance
- 07:22Conclusion
Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
