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Chapters
- 00:05Title
- 01:32Agar Bridge and Cut-open Rig Preparation
- 03:54Cut-open Procedure
- 09:48Results: The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique with Fluorometry
- 10:27Conclusion
The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.
