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该<em>爪蟾</em>卵母细胞切割开凡士林隙电压钳技术与荧光

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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

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该<em>爪蟾</em>卵母细胞切割开凡士林隙电压钳技术与荧光

DOI:

10.3791/51040-v

•

10:57 min

•

March 11, 2014

•

Michael W. Rudokas, Zoltan Varga, Angela R. Schubert, Alexandra B. Asaro, Jonathan R. Silva

¹Department of Biomedical Engineering,Washington University in St. Louis

Chapters

00:05Title
01:32Agar Bridge and Cut-open Rig Preparation
03:54Cut-open Procedure
09:48Results: The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique with Fluorometry
10:27Conclusion

Summary

자동 번역

剖切开凡士林差距的方法来获得电压依赖性离子通道的离子和门控电流的表达在非洲爪蟾卵母细胞具有高分辨率的快速通道动力学低噪音录音。有轻微的修改，电压钳荧光可以被耦合到所述剖切开的卵母细胞的协议。

Tags

Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Fluorometry Electrophysiology Ion Channels HNaV1.5 Two-electrode Voltage Clamp Fast Kinetics Voltage Clamp Fluorometry

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