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Imaging Denatureret Kollagen Strands<em> In vivo</em> Og<em> Ex vivo</em> Via Photo-udløst Hybridisering af Caged Collagen mimetiske peptider
JoVE 신문
생체공학
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JoVE 신문 생체공학
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatureret Kollagen Strands<em> In vivo</em> Og<em> Ex vivo</em> Via Photo-udløst Hybridisering af Caged Collagen mimetiske peptider

DOI:
10.3791/51052-v

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07:03 min

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January 31, 2014

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, , ,
1Department of Bioengineering,University of Utah, 2Department of Radiology and Radiological Science,Johns Hopkins University School of Medicine, 3Institute for NanoBiotechnology,Johns Hopkins University

Chapters

  • 00:05Title
  • 01:25Photo-activation and IV Injection of Caged Collagen Mimetic Peptides (CMPs)
  • 02:22Near Infrared (NIR) Fluorescence Imaging of Skeletal Collagen Remodeling Activity In vivo
  • 03:31Visualization of Collagens in Ex vivo Tissue Sections
  • 05:19Representative Images
  • 06:28Conclusion

Summary

자동 번역

자동 번역

Denne procedure in vivo nær-IR fluorescensimagografi af kollagen remodeling aktiviteter i mus såvel som ex vivo-farvning af collagener i vævssnit ved hjælp af bur kollagen mimetiske peptider, der kan være tilgængeligt udløses til at hybridisere med denaturerede kollagen strenge viser.

Tags

CollagenDenatured CollagenExtracellular MatrixCaged Collagen Mimetic PeptidePhoto-triggered HybridizationIn Vivo ImagingEx Vivo ImagingProteasesTriple Helix

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