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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis

Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis

DOI:
10.3791/51073-v

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10:55 min

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March 16, 2014

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,
1Department of Organismic and Evolutionary Biology,Harvard University

Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:07Introduction: Animal Culture
  • 03:11Procedure: Removing a Single Cell from the 8 Cell Stage during Parhyale Development
  • 04:37Gathering Embryos: Materials
  • 06:14Ablating g: Materials
  • 07:07Ablating g: Procedure
  • 10:18Representative Results

Summary

자동 번역

자동 번역

The amphipod Parhyale hawaiensis is a promising model organism for studies of crustacean embryology and comparative arthropod development and evolution. This protocol describes a method for manual removal of single blastomeres from early cleavage stage embryos of Parhyale.

Tags

Keywords: Parhyale HawaiensisAmphipod CrustaceanEight-cell EmbryoBlastomere AblationGerm Layer FateManual AblationHoloblastic CleavageModel OrganismDevelopmentCell Removal

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