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植物蛋白复合体的翻译后修饰的鉴定
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Identification of Post-translational Modifications of Plant Protein Complexes
DOI:

10:07 min

February 22, 2014

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Chapters

  • 00:05Title
  • 01:37N. benthamiana and A. thaliana Protein Samples
  • 03:24Protein Immunoprecipitation
  • 05:32Protein Digestion
  • 07:48Results: Identification of Post-translational Modifications of Tagged Proteins
  • 09:13Conclusion

Summary

자동 번역

我们在这里描述的一个协议,用于植物蛋白质复合物的纯化和表征。我们证明了在一个复杂的免疫沉淀一个单一的蛋白质,这样我们就可以识别其翻译后修饰及其相互作用的合作伙伴。

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