JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:38Effector CD8+ T Cell Isolation
- 03:48Target CD4+ T Cells Isolation
- 05:45Coculture
- 06:17Flow Cytometry Staining and Acquisition
- 06:58Results: Representative Flow Cytometry Cytotoxicity Assay
- 09:48Conclusion
This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.
