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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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환경과학
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JoVE 신문 환경과학
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

DOI:
10.3791/51117-v

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10:16 min

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January 16, 2014

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, , , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Soil Plant Nutrient REsearch,USDA-ARS, 3Department of Soil and Crop Sciences,Colorado State University

Chapters

  • 00:05Title
  • 01:26Chamber Construction
  • 02:37Temperature, Humidity, and CO2 Controls
  • 04:51Irrigation System and Potting Plants
  • 06:23Fertilization with a 15N-labeled Fertilizer
  • 07:22Harvest
  • 08:14Results: Successful Incorporation of 13C and 15N into Plant Tissue
  • 09:51Conclusion

Summary

자동 번역

자동 번역

This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

Tags

Keywords: 13C15NIsotope LabelingContinuous LabelingUniform LabelingDifferential LabelingPlant MaterialEcosystem ProcessesBiogeochemical TransformationsLitter DecompositionSoil Organic MatterStructural ComponentsMetabolic Components

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