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Chapters
- 00:05Title
- 01:37Preparation of Cell Lines
- 02:54Preparation of Animals and Experimental Setup
- 04:14Separation of Bladder Wall Layers and Intramural Inoculation of Bladder cancer Cells
- 05:54Representative Bladder Cancer Injection Results
- 07:39Conclusion
The established technique to inoculate primary invasive orthotopic bladder cancer xenografts requires laparotomy and mobilization of the bladder. This procedure inflicts significant morbidity on the mice, is technically challenging and time-consuming. We therefore developed a high-precision, percutaneous approach utilizing ultrasound guidance.
