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Chapters
- 00:05Title
- 01:21Infecting Cells with Chlamydia
- 03:04Live Cell Visualization of Chlamydia Inclusions
- 03:50Quantitation of Inclusion Growth Parameters in Live Cells
- 05:40Results: Chlamydia Vacuole Growth Dynamics in GFP-HeLa Cells
- 07:04Conclusion
A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.
