Journal
/
신경과학
/
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

DOI:
10.3791/51139-v

•

11:56 min

•

February 12, 2014

•

, , , ,
1Department of Anatomy & Neuroscience,The University of Melbourne, 2Department of Neurobiology,Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health,The University of Melbourne

Chapters

  • 00:05Title
  • 01:28Preparation of Dissociated Primary Embryonic Neuron Culture
  • 04:56Antibody Incubation with Live Neurons
  • 10:26Representative Imaging Results
  • 11:33Conclusion

Summary

자동 번역

자동 번역

We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.

Tags

Keywords: Differential LabelingCell-surface ProteinsInternalized ProteinsAntibody FeedingLive Cultured NeuronsTransmembrane ReceptorProtein TraffickingEndocytosisFluorescent Secondary AntibodySubcellular Localization

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/51139?article_type=v

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
check_url/kr/51139?article_type=v

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
check_url/kr/51139?article_type=v

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
check_url/kr/51139?article_type=v

Live Imaging of Dorsal Root Axons after Rhizotomy
check_url/kr/51139?article_type=v

Inducing Dendritic Growth in Cultured Sympathetic Neurons
check_url/kr/51139?article_type=v

Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
check_url/kr/51139?article_type=v

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
check_url/kr/51139?article_type=v

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
check_url/kr/51139?article_type=v

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
check_url/kr/51139?article_type=v

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Read Article